SAFETY IN THE USE OF LABORATORY EQUIPMENT - How Many Are You Familiar With?
Time：
2025-12-19 13:57
Laboratory equipment can be hazardous if they are not used and maintained properly. The following are some safety procedures for various instruments. It is recommended that all experimenters bookmark and familiarize themselves with these guidelines!
- Review and follow instructions provided in the manufacturer's manuals and standard operating procedures
- The manuals and procedures should be located with the equipment or in a location that is easily accessible to all laboratory personnel
- Maintenance or repairs to any laboratory equipment should only be performed by a qualified individual
- Safety devices on laboratory equipment must never be disabled or altered
CENTRIFUGE
Inspect the centrifuge tubes prior to use for stress lines, hairline cracks and chipped rims. Never operate a centrifuge if the rotor lid is missing its O-ring.
Before using centrifuge check that the correct rotors are being used and ensure it is properly balanced. Load the rotor with samples arranged symmetrically. Opposing tubes must be of equal weight. If necessary, use "water blank" tubes to balance sample tubes of unequal weight.
Unless fitted with a suitable exhaust system, do not centrifuge materials capable of creating flammable or explosive vapours.
Never exceed the specified speed limitations of the rotor. Do not attempt to move the centrifuge while it is in operation.
Do not open the lid during or immediately after operation, attempt to stop a spinning rotor by hand or with an object, or interfere with the interlock safety device.
Do not leave the centrifuge until it has reached its programmed speed. Immediately abort the run if you hear abnormal vibration, whining or grinding noises.
At the end of the run, ensure the rotor and centrifuge are cleaned according to the manufacturer's instructions. Do not use abrasive cleaners. Rotors are easily damaged. Do not use metal tools to remove tubes or clean.
OVEN, HOT PLATE AND HEATING MANTLE
Ovens are commonly used to remove water and solvents from samples, provide a stable elevated temperature and dry laboratory glassware. Hot plates are used to heat various laboratory solutions a heating mantles are used to heat reaction or sample solutions in round bottom flasks or reaction vessels.
Check for corrosion of thermostats. Make sure the electric power connections are made correctly and that the power cable is not damaged. Discontinue use of any heating mantle if the heating element is exposed. Hot plates or ovens with damaged thermostats are to be removed from service until repaired.
Samples should not be placed at the bottom of the working chamber, and samples on the shelves should not be too densely packed to avoid affecting airflow circulation.
Avoid heating toxic materials in an oven unless it is continuously vented outdoors. Flammable and volatile samples and organic solvents require an explosion-proof drying oven. Glassware that has been rinsed with an organic solvent should not be placed in an oven for drying; it must first be rinsed with distilled water or equivalent. When heating volatile, flammable liquids on a hot plate, do so only in a fume hood.
Prolonged operation of the drying oven at extreme temperatures is not recommended, as it can accelerate instrument aging and increase safety risks. When the temperature of heating devices rises above what is required, significant fire hazards can be created.
AUTOCLAVE
Inspect that the autoclave is not hot and all previous materials have been removed.
Make sure that the water inside the autoclave is at the optimal level. Ensure that the water level is at the optimum, fill in cold tap water if the water is less.
All samples must be wrapped in appropriate material. All containers and packaging must be made of autoclavable materials. Ensure that all pipes are tightly closed without leakage and lock the lid tightly.
Chemicals that are prone to explosion or sudden pressure increase upon contact with vapor media cannot be sterilized using autoclaves.
Switch on the power and allow the pressure to raise to the recommended level (normally @15ppi). Leave the autoclave on for sterilization for 15-20 minutes at the recommended pressure.
Switch of the power and allow the autoclave to cool off. Open only when the pressure is decreased to the minimum (Usually after 1 hour after switching off). Open the lid slowly to let off any stores pressure.
LAMINAR AIR FLOW
Before one hour of your work start the laminar air flow main power switch "ON". Then clean the platform of laminar air flow with 70% ethanol. Now switch on the UV light of laminar air flow for at least 45-60 minutes.
The operating area temperature must not exceed 60℃ to avoid damaging the HEPA filter.
Regularly inspect the HEPA filter; replace it when the airflow velocity is less than 0.32 m/s.
INCUBATOR
Hazards and risk associated with the use of incubator and precautions to be taken.
All containers/materials placed in incubator should be labelled with name, date and contents.
Since temperature changes can affect bacteria, it’s best to avoid causing the cabinet’s temperature to fluctuate by frequently opening the door.
Containers/materials should be removed after the appropriate time of incubation to prevent overcrowding or contamination of the incubator.
The interior should be cleaned often to stop the organisms from congregating on the shelves or the corners of the incubators.
Any spillage should be reported immediately to the laboratory technician/teacher and should be cleaned up in a manner appropriate to the material spilled.
Any faults associated with an incubator should be reported immediately to the laboratory technician/teacher. If an incubator is faulty, it should be switched off until repaired.
Incubator should be subject to servicing based on manufacturers recommendations.
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LICHEN Scientific Group
LACHOI Scientific Instrument (Shaoxing) Co., Ltd.
Add: Room 102, 1st Floor, Lianhe factory, No. 1423, Renmin East Road, Gaobu Street, Yuecheng District, Shaoxing City, Zhejiang Province, China
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